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Berna Demircan Tan

Istanbul Medeniyet University, Turkey

Title: Epigenetic alterations in high nitric oxide-adapted head and neck squamous cell carcinoma cell lines

Biography

Biography: Berna Demircan Tan

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) ranks as the sixth most frequent malignancy worldwide. A high level of nitric oxide (NO) has been identified in many types of human cancers including HNSCC. In this study, we investigated the eff ect of NO on promoter DNA methylation in cell lines of HNSCC.
Materials & Methods: Th e methylation statuses of the promoters of E-cadherin, RASSF1A and MGMT were analyzed in the parent and high nitric oxide (HNO) adapted cell lines of HNSCC using Illumina Mi Sequencing.
Results: We detected diff erence in promoter methylation levels of MGMT between the parent and HNO adapted cell lines. Methylation level was signifi cantly lower in SCC056-HNO cell line than its parent cell line SCC056 (p<0.0001), whereas it was higher in SCC016-HNO cell line compared to its parent cell line (p<0.0001). However, treatment of the cell lines with NO did not signifi cantly change methylation of E-cadherin and MGMT.
Conclusion: Our results suggest that NO may have diverse actions on DNA methylation. Further studies are required to clearly understand the role of NO in epigenetic alterations of HNSCC.